File:Cell-selective-knockout-and-3D-confocal-image-analysis-reveals-separate-roles-for-astrocyte-and-1742-2094-11-10-S1.ogv
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English: Contour-based 3D segmentation of luminal- and perivascular-associated cells in
Endo KO mice during EAE. The video shows 3D reconstruction of the representative Endo KO venule from Figure 7, allowing for enhanced visualization of the perivascular and luminal cellularity associated with the microvessel along x, y, and z axes. It further demonstrates the sequential steps employed for the contour-based 3D segmentation of DRAQ5+ cells into separate luminal and perivascular compartments, using 3D contour surfaces, as described in Materials and Methods. The nuclei were pseudo-colored in blue (luminal) and turquoise (perivascular). Each 180°-turn in the video indicates the following steps in sequence - 3D reconstruction → Lam1 (Red) isosurface → parenchymal BM contour → endothelial BM contour → segmented nuclei. |
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| Source | Video file from Paul D, Ge S, Lemire Y, Jellison E, Serwanski D, Ruddle N, Pachter J (2014). "Cell-selective knockout and 3D confocal image analysis reveals separate roles for astrocyte-and endothelial-derived CCL2 in neuroinflammation". Journal of Neuroinflammation. DOI:10.1186/1742-2094-11-10. PMID 24444311. PMC: 3906899. | ||
| Author | Paul D, Ge S, Lemire Y, Jellison E, Serwanski D, Ruddle N, Pachter J | ||
| Permission (Reusing this file) |
  This file is licensed under the Creative Commons Attribution 2.0 Generic license.
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 00:55, 3 November 2016 | 40 s, 1,024 × 1,024 (10.03 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Categories:
- Blood-brain barrier
- Brain microvascular endothelial cells
- Videos of central nervous system
- Experimental autoimmune encephalomyelitis
- 3D confocal imaging
- Chemokine CCL2
- Animal models of disease
- Three-dimensional imaging videos
- Knockout mice
- Confocal microscopic movies from Open Access Journals
- Myelin-oligodendrocyte glycoprotein
- Peptide fragments