File:Caveolae-Regulation-of-Mechanosensitive-Channel-Function-in-Myotubes-pone.0072894.s012.ogv

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Caveolae-Regulation-of-Mechanosensitive-Channel-Function-in-Myotubes-pone.0072894.s012.ogv (Ogg Theora video file, length 10 s, 512 × 256 pixels, 531 kbps, file size: 648 KB)

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English: A-CSD3 peptide causes Ca2+ influx associated with membrane detachment. Myotube cultures were loaded with Fluo4-AM, and DIC and fluorescent images were acquired every 3–4 min at 37°C. Cells were treated with 17 µM A-CSD3 at 12–15 min (∼frame 4) after which they rapidly took up Ca2+, contracted and released large amounts of membrane. The area of membrane released from the cells sometimes formed spheres with a diameter close to the longitudinal length of the original cell. This reinforces the fact that the membrane comprising the sarcolemma and t-tubule system is well in excess of the observable surface area of a myofiber [1]. Total video time = 117 min.
Date
Source Movie S4 from Huang H, Bae C, Sachs F, Suchyna T (2013). "Caveolae Regulation of Mechanosensitive Channel Function in Myotubes". PLOS ONE. DOI:10.1371/journal.pone.0072894. PMID 24023653. PMC: 3758351.
Author Huang H, Bae C, Sachs F, Suchyna T
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Date/TimeThumbnailDimensionsUserComment
current02:50, 14 September 201310 s, 512 × 256 (648 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P 131 kbps Completed 11:31, 21 August 2018 3.0 s
WebM 360P 211 kbps Completed 00:09, 30 November 2023 1.0 s
QuickTime 144p (MJPEG) 301 kbps Completed 06:12, 19 October 2024 1.0 s

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