File:Botulinum-Neurotoxins-A-and-E-Undergo-Retrograde-Axonal-Transport-in-Primary-Motor-Neurons-ppat.1003087.s007.ogv
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Botulinum-Neurotoxins-A-and-E-Undergo-Retrograde-Axonal-Transport-in-Primary-Motor-Neurons-ppat.1003087.s007.ogv (Ogg Theora video file, length 42 s, 509 × 63 pixels, 67 kbps, file size: 348 KB)
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[edit]DescriptionBotulinum-Neurotoxins-A-and-E-Undergo-Retrograde-Axonal-Transport-in-Primary-Motor-Neurons-ppat.1003087.s007.ogv |
English: HCE undergoes fast retrograde transport in non acidic organelles. Motor neurons cultures were incubated with fluorescent HCE (green) and Lysotracker (red) for 30 min at 37°C under depolarising conditions (60 mM KCl). Cells were then washed and imaged. HCE is retrogradely transported in organelles lacking Lysotracker. As discussed in the text, some of the carriers containing HCE (green) show a discontinuous transport in the retrograde direction (from left to right), whereas some others undergo anterograde transport. Cell body is out of view on the right. Frames were taken every 4 s and played at 5 frames/s. This movie is a representative example of experiments performed on two independent primary motor neuron cultures, replicated twice. |
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Source | Movie S4 from Restani L, Giribaldi F, Manich M, Bercsenyi K, Menendez G, Rossetto O, Caleo M, Schiavo G (2012). "Botulinum Neurotoxins A and E Undergo Retrograde Axonal Transport in Primary Motor Neurons". PLOS Pathogens. DOI:10.1371/journal.ppat.1003087. PMID 23300443. PMC: 3531519. | ||
Author | Restani L, Giribaldi F, Manich M, Bercsenyi K, Menendez G, Rossetto O, Caleo M, Schiavo G | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 17:01, 21 January 2013 | 42 s, 509 × 63 (348 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Restani L, Giribaldi F, Manich M, Bercsenyi K, Menendez G, Rossetto O, Caleo M, Schiavo G |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
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Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2012-12 |