File:Bicistronic-Lentiviruses-Containing-a-Viral-2A-Cleavage-Sequence-Reliably-Co-Express-Two-Proteins-pone.0020553.s004.ogv
Bicistronic-Lentiviruses-Containing-a-Viral-2A-Cleavage-Sequence-Reliably-Co-Express-Two-Proteins-pone.0020553.s004.ogv (Ogg multiplexed audio/video file, Theora/Vorbis, length 45 s, 640 × 480 pixels, 1.05 Mbps overall, file size: 5.66 MB)
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[edit]DescriptionBicistronic-Lentiviruses-Containing-a-Viral-2A-Cleavage-Sequence-Reliably-Co-Express-Two-Proteins-pone.0020553.s004.ogv |
English: Optokinetic behavior of 8-day-old GUCY1*B chicken that had been treated with pFIN-EF1-GFP-2A-GC1-WPRE lentivirus on embryonic day 2. This treated animal, unlike untreated animals, exhibited a robust, optokinetic response that was assigned a score of 3.0. Reflex responses were elicited by the high-contrast vertical square wave grating (bar width 1.25 cm) when rotated in either clockwise or counter clockwise. No responses were elicited by the solid white control stimulus. |
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Source | Video S3 from Verrier J, Madorsky I, Coggin W, Geesey M, Hochman M, Walling E, Daroszewski D, Eccles K, Ludlow R, Semple-Rowland S (2011). "Bicistronic Lentiviruses Containing a Viral 2A Cleavage Sequence Reliably Co-Express Two Proteins and Restore Vision to an Animal Model of LCA1". PLOS ONE. DOI:10.1371/journal.pone.0020553. PMID 21647387. PMC: 3103589. | ||
Author | Verrier J, Madorsky I, Coggin W, Geesey M, Hochman M, Walling E, Daroszewski D, Eccles K, Ludlow R, Semple-Rowland S | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 13:11, 18 November 2012 | 45 s, 640 × 480 (5.66 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Verrier J, Madorsky I, Coggin W, Geesey M, Hochman M, Walling E, Daroszewski D, Eccles K, Ludlow R, Semple-Rowland S |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Optokinetic behavior of 8-day-old GUCY1*B chicken that had been treated with pFIN-EF1-GFP-2A-GC1-WPRE lentivirus on embryonic day 2. This treated animal, unlike untreated animals, exhibited a robust, optokinetic response that was assigned a score of 3.0. Reflex responses were elicited by the high-contrast vertical square wave grating (bar width 1.25 cm) when rotated in either clockwise or counter clockwise. No responses were elicited by the solid white control stimulus. |
Software used | |
Date and time of digitizing | 2011 |
Language | English |
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2011
application/ogg
ed3a79f23b3b297d361c9f03903cdfac72ad2fe1
5,937,682 byte
45.30266666666667 second
480 pixel
640 pixel
- Molecular cell biology
- Genetic predispositions to diseases
- Videos of gene therapy
- Inherited eye disorders
- Videos of disorders of choroid and retina
- Chicks
- Videos of chickens
- Animal models of disease
- Viral genes
- Genetic vectors
- Guanylate cyclase
- HEK293 cells
- Leber's congenital amaurosis
- Lentivirus
- Luminescent proteins
- Genetic promoter regions
- Cell surface receptors
- Recovery of function
- Videos of retinas
- Transgenes
- Ocular vision
- Media from PLOS ONE
- Cleavage (embryo)