File:A-high-throughput-chemically-induced-inflammation-assay-in-zebrafish-1741-7007-8-151-S3.ogv

English: Supplementary Movie 2. Leukocytes patrol among neuromast cells after copper induced damage. Description: Compound cldnB::GFP, lysC::DsRED2 transgenic fish that have green-labeled neuromasts and red-labeled leukocytes were treated with 50 μM CuSO4 and immediately mounted for imaging for 1 hour under a confocal microscope. Images were taken in both channels and in bright-field illumination every 60 seconds in three z-planes for 1 hour and were then combined for every time point to produce the movie. Note the progressive disorganization of the neuromast cells, which loses its rosette structure while cells become detached from one another and leukocytes actively migrate throughout the organ. Original magnification, ×40.