File:A-Role-for-the-Chaperone-Complex-BAG3-HSPB8-in-Actin-Dynamics-Spindle-Orientation-and-Proper-pgen.1005582.s021.ogv
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[edit]DescriptionA-Role-for-the-Chaperone-Complex-BAG3-HSPB8-in-Actin-Dynamics-Spindle-Orientation-and-Proper-pgen.1005582.s021.ogv |
English: Related to Fig 7D —siBAG3-treated cells show spectacular blebbing of the mitotic cortex along with displacement of the mitotic spindle pushed into the bleb. Video microscopy of a representative HeLa-RFP-H2B cell transfected with siBAG3 and transduced with BacMam-GFP-a-tubulin and RFP-actin; cells were imaged for a 1 h-time period at ~1.5 min intervals using a Perkin Elmer UltraVIEW Spinning Disc Confocal and 40x air 0.75NA objective and single plane images are displayed at 7 frames/sec. Please note that RFP-H2B expression is variable in this cell line. |
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Source | S15 Movie from Fuchs M, Luthold C, Guilbert S, Varlet A, Lambert H, Jetté A, Elowe S, Landry J, Lavoie J (2015). "A Role for the Chaperone Complex BAG3-HSPB8 in Actin Dynamics, Spindle Orientation and Proper Chromosome Segregation during Mitosis". PLOS Genetics. DOI:10.1371/journal.pgen.1005582. PMID 26496431. PMC: 4619738. | ||
Author | Fuchs M, Luthold C, Guilbert S, Varlet A, Lambert H, Jetté A, Elowe S, Landry J, Lavoie J | ||
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 12:48, 8 December 2015 | 4.4 s, 720 × 720 (313 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Related to Fig 7D —siBAG3-treated cells show spectacular blebbing of the mitotic cortex along with displacement of the mitotic spindle pushed into the bleb. |
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Author | Fuchs M, Luthold C, Guilbert S, Varlet A, Lambert H, Jetté A, Elowe S, Landry J, Lavoie J |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | Video microscopy of a representative HeLa-RFP-H2B cell transfected with siBAG3 and transduced with BacMam-GFP-a-tubulin and RFP-actin; cells were imaged for a 1 h-time period at ~1.5 min intervals using a Perkin Elmer UltraVIEW Spinning Disc Confocal and 40x air 0.75NA objective and single plane images are displayed at 7 frames/sec. Please note that RFP-H2B expression is variable in this cell line. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2015-10-23 |