File:A-Quantitative-Comparison-of-Human-HT-1080-Fibrosarcoma-Cells-and-Primary-Human-Dermal-Fibroblasts-pone.0081689.s012.ogv

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A-Quantitative-Comparison-of-Human-HT-1080-Fibrosarcoma-Cells-and-Primary-Human-Dermal-Fibroblasts-pone.0081689.s012.ogv (Ogg Theora video file, length 4.8 s, 500 × 250 pixels, 458 kbps, file size: 268 KB)

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English: Treatment with matrix metalloproteinase inhibitor (GM6001) blocks migration for HT-1080 fibrosarcoma cells in synthetic ECM. Time-lapse images (15 min / frame) illustrating HT-1080s migrating in synthetic ECM (220 Pa, 1000 μM CRGDS) after treatment with DMSO (control, left) or matrix metalloproteinase (MMP) inhibitor (GM6001, right). Images represent minimum intensity z-projections (middle 500 μm of ~1000 μm thick matrix). HT-1080s are shown for the same time frame used for tracking and quantification of motility. No HT-1080s moved more than one cell length after treatment with MMP-inhibitor, demonstrating that proteolysis is required for migration in synthetic ECM. Time shown in Hr:Min. Scale bar = 100 μm.
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Source Movie S1 from Schwartz M, Rogers R, Singh S, Lee J, Loveland S, Koepsel J, Witze E, Montanez-Sauri S, Sung K, Tokuda E, Sharma Y, Everhart L, Nguyen E, Zaman M, Beebe D, Ahn N, Murphy W, Anseth K (2013). "A Quantitative Comparison of Human HT-1080 Fibrosarcoma Cells and Primary Human Dermal Fibroblasts Identifies a 3D Migration Mechanism with Properties Unique to the Transformed Phenotype". PLOS ONE. DOI:10.1371/journal.pone.0081689. PMID 24349113. PMC: 3857815.
Author Schwartz M, Rogers R, Singh S, Lee J, Loveland S, Koepsel J, Witze E, Montanez-Sauri S, Sung K, Tokuda E, Sharma Y, Everhart L, Nguyen E, Zaman M, Beebe D, Ahn N, Murphy W, Anseth K
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current05:08, 20 December 20134.8 s, 500 × 250 (268 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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VP9 240P 133 kbps Completed 21:43, 15 August 2018 2.0 s
WebM 360P 208 kbps Completed 12:48, 16 November 2023 1.0 s
QuickTime 144p (MJPEG) 341 kbps Completed 09:15, 14 October 2024 1.0 s

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