File:A-Bispecific-Antibody-Promotes-Aggregation-of-Ricin-Toxin-on-Cell-Surfaces-and-Alters-Dynamics-of-pone.0156893.s012.ogv

English: Live cell imaging of ricin-FITC in the presence of JJX12 and late endosomes. Real time imaging of A549 cells visualizing ricin-FITC in green pre-mixed with JJX12 prior to adding to cells and late endosomes in red labeled with a transient transfection as described in Material and Methods. The total fluorescence for the late endosomes marker is always overwhelming compared to ricin-FITC and therefore the absolute co-localization between ricin-FITC and the late endosomes is not apparent in the raw images prior to processing. Real time images were processed prior to quantification as described in Materials and Methods to determine co-localization values, which are reported in Fig 4C. Total length of the time lapse is 90 min.