File:41467 2024 49602 Fig2.webp

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Morphology and activity of methane-oxidizing bacteria under hypoxic and anoxic conditions

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English: Morphology and activity of methane-oxidizing bacteria under hypoxic and anoxic conditions.
a CARD FISH images of gammaproteobacterial MOB stained with probes Mγ84 and Mγ705 (red) and NC10 bacteria (Methylomirabilota) stained with probe DBACT−1027 (green). All cells were counterstained with 4′,6-diamidino-2-phenylindole (DAPI; blue). Crenothrix-like filaments were identified based on their morphology after DAPI staining. Gamma-MOB were classified based on their size and shape as large rods, cocci, and small rods (from left to right). Scale bar represents 3 µm.
b Excess 13C at.% enrichment of MOB after incubation with 13C-labeled methane for 24 h under hypoxic (red) and anoxic (blue) conditions determined by nanoSIMS analysis. 13C at.% values are given as excess values for each analyzed cell. The 0 value on the y-axis depicts the natural abundance of 13C (1.1 %). The number of cells analyzed per category (n) is shown as scatter and indicated above each boxplot. Boxplots depict the 25–75 % quantile range, with the center line representing the median (50 % quantile) and whiskers representing the 5 and 95 percentile. All analyses were performed on samples from 135 m water depth.
Date
Source Per­sis­tent ac­tivi­ty of ae­ro­bic me­tha­ne-oxi­di­zing bac­te­ria in an­oxic lake wa­ters due to me­ta­bo­lic ver­sa­ti­li­ty. In: Nature Com­munications, Volume 15, Article number 5293; doi:10.1038/s41467-024-49602-5
Author Sina Schorn, Jon S. Graf, Sten Littmann, Philipp F. Hach, Gaute Lavik, Daan R. Speth, Carsten J. Schubert, Marcel M. M. Kuypers, Jana Milucka
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current11:23, 1 September 2024Thumbnail for version as of 11:23, 1 September 20241,501 × 1,092 (68 KB)Ernsts (talk | contribs)Uploaded a work by Sina Schorn, Jon S. Graf, Sten Littmann, Philipp F. Hach, Gaute Lavik, Daan R. Speth, Carsten J. Schubert, Marcel M. M. Kuypers, Jana Milucka from ''Per­sis­tent ac­tivi­ty of ae­ro­bic me­tha­ne-oxi­di­zing bac­te­ria in an­oxic lake wa­ters due to me­ta­bo­lic ver­sa­ti­li­ty.'' In: ''Nature Com­munications'', Volume 15, Article number 5293; doi:10.1038/s41467-024-49602-5 with UploadWizard

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