File:. Neurogenesis in the lateral line placodes of Q-VD-OPh-treated mice.jpg

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Description Fig. 5. Neurogenesis in the lateral line placodes of Q-VD-OPh-treated mice. (A,C,F,G) Reconstructions of serially sectioned mouse embryos (section interval evaluated=10 μm) show ectoderm (light grey), otic vesicle with detachment site (dark grey), epibranchial placodes (orange), lateral line placodes (blue), immunopositive cells (brown dots), developing cranial nerves (partly numbered brown contours in G). (B,D,E) Micrographs taken from serially sectioned mouse embryos. (A) Sox2 + cells are present in epibranchial placodes and, to a much lesser extent, in lateral line placodes (embryo #021). (B) Sox2+ mantle (m) and support (s) cells in a neuromast primordium of a middle lateral line placode. Note the apical cavity (arrowhead). (C) Ngn1 + cells are present in epibranchial placodes and, to a much lesser extent, in lateral line placodes (embryo #039, mirror-imaged right side). (D) Ngn1 + neuroblasts in an anterodorsal lateral line placode. (E,F) Ngn2 + neuroblasts are present in epibranchial placodes, but absent from lateral line placodes (embryo #001). (G) Tubb3+ neurons constitute the anlagen of the cranial nerves 7 and 8 (VII/VIII), 9 (IX), 10 (X), 11 (XI) and 12 (XII). Tubb3+ candidates for vestigial lateralis ganglia (asterisks) reside in close proximity to the middle lateral line placode (embryo #014). All micrographs were adjusted for brightness (including slight gamma changes), colour balance, and sharpness. Scale bars: 5 μm. ad, m, p, anterodorsal, middle, and posterior lateral line placode, respectively; e1, e2, e3, epibranchial placodes 1, 2, 3, respectively; ot, otic anlage; ov, optic vesicle; Q-VD-OPh, pan-caspase inhibitor; wec, whole embryo culture.
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Source https://cob.silverchair-cdn.com/cob/content_public/journal/bio/7/6/10.1242_bio.031815/4/bio031815.pdf?Expires=1733434658&Signature=Vux2hqZa1-GF1SDs1AEeEzshbHrS4RanPB7vS9WAX-RzFIMgc3M0UCgDh6c0fJvO7eNPV2rmxhL5HJZZSQw7LbrUMLDGUF92J2nvFp1GmRZQw-gZzN8jQBuHPU1a6HqwGAzk-3zUGJh0jaqfTNr3OK2RzVsXGa2WqzEabG0zWbWAS57KN8ln1bMU198Q0X-fGaOQ9uCgiM6Nz254OrABlBYqRo~5s2TkQINYN7jjWJ7CoRQvoKWBKqOnh0s4ZWgFP8o~~hBl6BKPtxwJ55bs92FA~dFiYV4Bjv~4AI7MrRCvlTHctMtcNJ8yrVjIbpwlNYboJ~ADo1omcg65Vb3BQw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA Stefan Washausen and Wolfgang Knabe. Lateral line placodes of aquatic vertebrates are evolutionarily conserved in mammals. Biology Open (2018) 7, bio031815. doi:10.1242/bio.031815
Author Stefan Washausen and Wolfgang Knabe
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current21:51, 31 October 2024Thumbnail for version as of 21:51, 31 October 2024714 × 820 (363 KB)Rasbak (talk | contribs){{Information |description=Fig. 5. Neurogenesis in the lateral line placodes of Q-VD-OPh-treated mice. (A,C,F,G) Reconstructions of serially sectioned mouse embryos (section interval evaluated=10 μm) show ectoderm (light grey), otic vesicle with detachment site (dark grey), epibranchial placodes (orange), lateral line placodes (blue), immunopositive cells (brown dots), developing cranial nerves (partly numbered brown contours in G). (B,D,E) Micrographs taken from serially sectioned mouse embr...

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