File:The Biological bulletin (20370835372).jpg

Title: The Biological bulletin
Identifier: biologicalbullet191mari (find matches)
Year: [1] (s)
Authors: Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983
Subjects: Biology; Zoology; Biology; Marine Biology
Publisher: Woods Hole, Mass. : Marine Biological Laboratory
Contributing Library: MBLWHOI Library
Digitizing Sponsor: MBLWHOI Library

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CELL BIOLOGY 271 actin fibers (in this case 80 filaments). Rsing;C in artificial seawa- ter is estimated as 0.0107 ± 0.0013 nm (n = 5). This value is a rough estimate because the actual number of actin filaments in the bundles used in this study was not determined, but the value should be useful tor estimating the number of actin fil- aments contained in cells observed with the new pol-scope. We are indebted to Dr. L. G. Tilney and S. Inoue for their invaluable suggestions. This work is supported by 1996 Lilli Fellowship awarded to P.O. and NIH grant (RO1 GM49210) to R.O. Literature Cited 1. Oldenboure, R. 1996. Nature 381: 811-812. 2. Tilney, L. G. 1975. J. CellBiol. 64: 289-310. 3. Brokaw, C. J. 1995. Methods Cell Biol.47: 231-238. 4. Tran, P., S. Inoue, E. D. Salmon, and R. Oldenbourg. 1995. Bull 189:206. Biol. Reference: Biol. Bull. 191: 271-272. (October, 1996) Mitotic Spindle Fine Structures Observed With Polarized Light Phong Tran, E. D. Salmon, and Rudolj Oldenbourg (Marine Biological Laboratory) Recent advances and improvements on the polarizing light microscope have allowed for noninvasive imaging of cellular fine structures with high sensitivity and resolution irrespective of specimen orientation (1,2). We have used our modified po- larized light microscope (pol-scope) to image newt lung epithe- lial cells during mitosis to examine the dynamic of spindle fine structures. Primary cultures of newt (Taricha granulosa) lung epithelial cells were prepared as described by Rieder and Hard (3). The cells were then imaged with the pol-scope equipped with a Ni- kon PlanApo 60X/1.4NA low-strain objective and a matching Nikon Universal 1.4NA condenser. Figure 1 shows time-lapsed images of one mitotic spindle. Along with the strongly birefringent arrays of microtubules nu-
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Figure 1. The birefringence of mitotic spindle fine structures imaged with the pol-scope. Sequences a-f depict spindle dynamics over time. The spindle microtubules, chromosomes, and kinetochore fibers are clearly observable. Sequences a-c depict the position of the lone chromosome. As the chromosome (*) advances to the mid-zone, the birefringent intensity of the trailing microlubules decreases to match the intensity of the opposite half. Note the indentation impinging on the chromosome arm fb). Sequences d-fdepict a kinetochore fiber (A/ Slight buckling of the fiber is observable concurrent with the movement of the kmetochore/chromosome away from the pole. Note that the indentation of the kinetochore is visible as the fiber moves away from the pole (d.f). but not as it moves toward the pole (e). Bar =10 ^m.

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