File:The Biological bulletin (1960) (20380514785).jpg

Title: The Biological bulletin
Identifier: biologicalbullet11819lill (find matches)
Year: 1899 (1890s)
Authors: Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-; Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report; HighWire Press
Subjects: Biology; Zoology; Marine biology
Publisher: Lancaster, Pa. (etc. ) : Lancaster Press, inc. (etc. )
Contributing Library: Smithsonian Libraries
Digitizing Sponsor: Biodiversity Heritage Library

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HORMONES AND RETINAL PIGMENT 401 Digestion of the light-adapting and dark-adapting hormones by trypsin The object of the first experiment of this series was to demonstrate that a boiled solution of trypsin would not inactivate the retinal pigment hormones. After boiling, the enzyme would have been inactivated but some contaminant might have possibly destroyed the hormones. This possibility had to be ruled out. For the first experiment of this group a 2% solution of trypsin in sea water was prepared; at the same time a freshly prepared extract of eight eyestalks in 0.2 ml. sea water was boiled for 30 seconds and centrifuged. Equal volumes of the boiled trypsin solution and the eyestalk extract were mixed together. The
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Figure 4. The distal retinal pigment index 30 minutes after prawns had been taken from darkness and placed on a white background under an illumination of 31 ft. c, versus the number of days the specimens had been in darkness. mixture was then injected into one-eyed prawns in black pans under an illumina- tion of 22 ft. c. Boiled trypsin solution diluted with an equal volume of sea water was injected into the controls. The results were those that were anticipated. A light-adaptational response occurred and lasted two hours. A dark-adaptational response followed. The extent of the latter was not followed to its completion. Fingerman, Lowe and Sundararaj (1959j reported that the dark-adaptational response lasted at least five hours. The experiment was performed two more times and the results were

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