File:Summary of proteomic approaches utilized in the study of stem cells..jpg

English: Advantages and disadvantages of the various methods that have been used in proteomic profiling and protein interactome mapping are highlighted. A. Two dimensional electrophoresis (2-DE) followed by mass spectrometry (MS) analysis has been widely used to compare various populations of stem cells to more differentiated cell types. B. iTRAQ (isobaric tags for relative and absolute quantification) and ICAT (isotope coded affinity tags) are two chemical labeling approaches that have been used prior to tandem mass spectrometry (MS/MS) analysis in comparison of purified stem cell populations. C. SILAC (stable isotope labeling with amino acids in culture) is a metabolic labeling approach that has been used in proteomic profiling and quantitative phosphoproteomic studies. D. Affinity-based purification of protein complexes followed by MS/MS analysis has been widely used in protein interactome studies. E. The use of functional protein arrays is a very promising approach that has predominantly been utilized in yeast protein interactome studies.