File:Schematic depiction of LC-MS MS procedure..jpg

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Schematic_depiction_of_LC-MS_MS_procedure..jpg (661 × 600 pixels, file size: 76 KB, MIME type: image/jpeg)

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English: Protein extracts are made from stem cells of any type and first fractionated by SDS-PAGE. Individual bands or the whole lane are then subjected to in situ digestion with trypsin. After trypsin digestion, the product is subjected to either single-dimensional (LC) or multi-dimensional (LC/LC) liquid chromatography for further separation of the mixture. Elute from LC is then ionized by electrospray ionization, and each elute peak first passes through an MS (typically MS2) for mass determination. Each peak is then separated in MS1 and passes into a collision chamber where it is further fragmented and subsequently analyzed in MS2, which aids in peptide sequence determination.
Date Published July 14, 2008.
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StemBook Figure 2 Schematic depiction of LC-MS MS procedure.

  • Wang, J., Trowbridge, J.J., Rao, S. and Orkin, S.H., Proteomic studies of stem cells (July 14, 2008), StemBook, ed. The Stem Cell Research Community, StemBook, doi/10.3824/stembook.1.4.1, http://www.stembook.org.
Author Wang, J., Trowbridge, J.J., Rao, S. and Orkin, S.H., Proteomic studies of stem cells (July 14, 2008), StemBook, ed. The Stem Cell Research Community, StemBook, doi/10.3824/stembook.1.4.1, http://www.stembook.org.
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