File:Journal.pbio.0030157.g001a.jpg

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Deutsch: Zellkern eines menschlichen Fibroblasten, in dem alle 24 verschiedenen Chromosomen (1 - 22, X und Y) per Fluoreszenz in situ Hybridisierung (FISH) mit einer unterschiedlichen Kombination von insgesamt 7 Fluorochromen angefärbt wurden. Gezeigt ist eine mittlere Ebene in einem deconvolvierten Bildstapel, der mit Weitfeld-Mikroskopie aufgenommen wurde.

English: Top: FISH (Fluorescence in situ hybridization) labeling of all 24 different human chromosomes (1 - 22, X, and Y) in a fibroblast nucleus, each with a different combination of in total seven fluorochromes. Shown is a mid-plane of a deconvoluted image stack which was recorded by wide-field microscopy.

Date

26 April 2005

Source

Bolzer et al., (2005) Three-Dimensional Maps of All Chromosomes in Human Male Fibroblast Nuclei and Prometaphase Rosettes. PLoS Biol 3(5): e157 DOI: 10.1371/journal.pbio.0030157, part of Figure 1.

Author

Andreas Bolzer, Gregor Kreth, Irina Solovei, Daniela Koehler, Kaan Saracoglu, Christine Fauth, Stefan Müller, Roland Eils, Christoph Cremer, Michael R. Speicher, Thomas Cremer

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	Date/Time	Thumbnail	Dimensions	User	Comment
current	22:37, 18 March 2017		1,367 × 1,225 (148 KB)	Dietzel65 (talk \| contribs)	{{Information \|Description= {{de\|Zellkern eines menschlichen Fibroblasten, in dem alle 24 verschiedenen Chromosomen (1 - 22, X und Y) per Fluoreszenz in situ Hybridisierung (FISH) mit einer unterschiedlichen Kombination von insgesamt 7 Fluorochromen...

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- Fluoreszenzmikroskopie

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Copyright holder	Creative Commons Attribution License
Image title	<title>24-Color 3D FISH Representation and Classification of Chromosomes in a Human G0 Fibroblast Nucleus</title><p>(A) A deconvoluted mid-plane nuclear section recorded by wide-field microscopy in eight channels: one channel for DAPI (DNA counterstain) and seven channels for the following fluorochromes: diethylaminocoumarin (Deac), Spectrum Green (SG), and the cyanine dyes Cy3, Cy3.5, Cy5, Cy5.5, and Cy7. Each channel represents the painting of a CT subset with the respective fluorochrome. The combinatorial labeling scheme is described in<xref ref-type="sec" rid="s4">Materials and Methods</xref>. RGB images of the 24 differently labeled chromosome types (1–22, X, and Y) were produced by superposition of the seven channels (bottom right).</p><p>(B) False color representation of all CTs visible in this mid-section after classification with the program goldFISH.</p><p>(C) 3D reconstruction of the complete CT arrangement in the nucleus viewed from different angles.</p><p>(D) Simulation of a human fibroblast model nucleus according to the SCD model (see<xref ref-type="sec" rid="s4">Materials and Methods</xref>). The first image shows 46 statistically placed rods representing the 46 human chromatids. The next three images simulate the decondensation process and show the resulting CT arrangement obtained after different numbers of Monte Carlo relaxation steps (200, 1,000, and 400,000). This set of figures is taken from<xref ref-type="supplementary-material" rid="sv001">Video S1</xref>.</p>
Publisher	Public Library of Science
Short title	Figure 1
Source	info:doi/10.1371/journal.pbio.0030157
Width	2,790 px
Height	2,910 px
Bits per component	8 8 8
Compression scheme	LZW
Pixel composition	RGB
Orientation	Normal
Number of components	3
Horizontal resolution	600 dpi
Vertical resolution	600 dpi
Data arrangement	chunky format
Software used	Adobe Photoshop CS2 Windows
File change date and time	23:22, 18 March 2017
Color space	Uncalibrated
Image width	1,367 px
Image height	1,225 px
Date(s)	26 April 2005
Date and time of digitizing	00:22, 19 March 2017
Date metadata was last modified	00:22, 19 March 2017
Identifier	info:doi/10.1371/journal.pbio.0030157.g001
IIM version	2

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