File:GFP fluorophore formation.svg

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Description
English: Formation of the GFP fluorophore (green) from the Ser65-Tyr66-Gly67 tripeptide. In a first step (not shown), protein folding aids in positioning the Ser carbonyl and Gly nitrogen. Then, cyclization occurs at aforementioned moieties and water is eliminated. Finally, oxidation / desaturation occurs on Tyr, leading to formation of a large pi-delocalized system (green).

Reference: Reid BG, Flynn GC (1997). "Chromophore Formation in Green Fluorescent Protein". Biochemistry 36 (22): 6786–6791. DOI:10.1021/bi970281w. PMID 9184161.

Other models of fluorophore formation exist, see for example doi:10.1021/bi8007164
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Author Hbf878

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current22:39, 9 January 2019Thumbnail for version as of 22:39, 9 January 2019512 × 83 (27 KB)Hbf878 (talk | contribs)User created page with UploadWizard

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