File:Fluorescence Imaging 02.jpg
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Deutsch: Fluoreszenzbildgebung eines orthotopen Implantates eines Pankreaskarzinoms eine Maus. Die Bildgebung dient zu Bestimmung des Tumorvolumens. Die Maus wurde zwei Wochen nach der Injektion von humanen Pankreastumorzellen vom Typ XPA-1 fotografiert. Als Farbstoff wurde rot-fluoreszierendes Protein verwendet (RFP). Die Bildreihe A-C zeigt die betäubte Maus. Bild A ist ein Fusionsbild aus sichtbarem Licht und Fluoreszenzaufnahme. Bild B zeigt die Fluoreszenz des mit RFP markierten Tumors (nicht quantitativ). Bild C ist eine monochrome quantitative Aufnahme der Fluoreszenz des Tumors aus Bild A+B. In Reihe D-F wurde die Bauchdecke der Maus geöffnet und die Aufnahmen von A biC wiederholt. Deutlich zu erkennen ist die bessere Auflösung der Bilder, da die störenden Einflüsse der Bauchdecke fehlen.
English: Fluorescence imaging of orthotopic pancreatic tumor implants and measurement of tumor size. This mouse was imaged two weeks after orthotopic injection of a human pancreatic cell line (XPA-1) stably expressing red fluorescent protein (RFP). A-C. Evaluation of tumor size by whole body imaging of an anesthetized mouse. A. Bright field and color fluorescence images were overlaid to show the coronal image of the fluorescent tumor. B. Enlarged whole body fluorescence image of tumor. This image was obtained using a color (non-quantitative) fluorescence imaging camera. C. Monochrome (quantitative) fluorescence image of the tumor shown in panels A and B. This coronal image was analyzed using ImageJ software to determine the cross-sectional size of the tumor in millimeters squared (mm2). D-F. Evaluation of tumor size at necropsy. D. Overlay of bright field and color fluorescence images showing the fluorescent tumor implant at necropsy. E. Enlarged color fluorescence-only image of the tumor as shown in D. F. As for panel C above, the cross-sectional size of the tumor shown in panels D and E was determined using ImageJ software. |
| Date | published 8 April 2009 |
| Source | Cynthia S Snyder, Sharmeela Kaushal, Yuko Kono, Hop S Tran Cao, Robert M Hoffman and Michael Bouvet: Complementarity of ultrasound and fluorescence imaging in an orthotopic mouse model of pancreatic cancer. In: BMC Cancer 2009, 9:106 doi:10.1186/1471-2407-9-106 Open Access |
| Author | Cynthia S Snyder, Sharmeela Kaushal, Yuko Kono, Hop S Tran Cao, Robert M Hoffman and Michael Bouvet |
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| current | 20:07, 22 June 2010 | | 928 × 619 (187 KB) | Kuebi (talk | contribs) | {{Information |Description={{de|Fluoreszenzbildgebung eines orthotopen Implantates eines Pankreaskarzinoms eine Maus. Die Bildgebung dient zu Bestimmung des Tumorvolumens. Die Maus wurde zwei Wochen nach der Injektion von humanen Pankreastumorzellen vom T |
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