File:Ciba Foundation Symposium on the Regulation of Cell Metabolism (1959) (20424296389).jpg

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Title: Ciba Foundation Symposium on the Regulation of Cell Metabolism
Identifier: cibafoundationsy1959ciba (find matches)
Year: 1959 (1950s)
Authors: Ciba Foundation Symposium on the Regulation of Cell Metabolism (1958 : London, England); Wolstenholme, G. E. W. (Gordon Ethelbert Ward); O'Connor, Cecilia M. (Cecilia Mary), 1927-
Subjects: Cell metabolism
Publisher: Boston, Little, Brown
Contributing Library: MBLWHOI Library
Digitizing Sponsor: MBLWHOI Library

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162 F. Dickens, G. E. Clock and P. McLean The 12 TPNH so formed could carboxylate 12 moles of pyruvate: Pyruvate- + TPNH + CO2 ^ malate^- + TPN+ thus yielding, by the "by-pass", 12 PEP and so eventually 6 moles of glucose: ratio of glucose resynthesized/glucose oxidized = 6/1. The possibility of this coupling of a TPNH-dependent MB t-0-5 "F6Pl-^ 2PEP —^ F6P+ HPO4" AG'-6/moIe F6P 6 Pentose P -fH20 ^5F6P + HPO4 AG'-2/mole F6P ITP DPNH, IDP t PEP ILACTATEI
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A ('^.( PentoseP +CO2 1 and I w ^ 6PG Qv. AG -3-5/mole approx. Fig. 4. Coupling of HMP oxidative pathway with carboxylation of pyruvate by means of TPNH. The numbers on arrows indicate approximate value of AG' for the reactions shown (cf. Fig. 2). reductive carboxylation with reversal of glycolytic reactions requires the presence of G6P- and 6-PG-dehydrogenases as well as TPN in the tissue concerned. This is the case in liver, a tissue in which randomization of pyruvate carbon atoms in the glucose of the glycogen synthesized accords well with an indirect route of pyruvate incorporation, in which carboxyla- tion to give a stage involving a symmetrical dicarboxylic acid

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