File:Рис. 8. Применение метода FRET для обнаружения мембранного потенциала.jpg
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Русский: Применение метода FRET для обнаружения мембранного потенциала. В данном примере неспособный к проникновению через мембрану донорный флуорофор расположен на внешней стороне клеточной мембраны, прикрепленный к фосфолипиду. Заряженный трансмембранный оксонольный акцептор предпочтительно связывается с той же внешней стороны в случае полностью поляризованной покоящейся клетки – тогда из-за близкого расположения флуорофоров образуется FRET-пара. При деполяризации происходит транслокация оксонола к внутренней стороне, что сопровождается увеличением испускания группы донора по сравнению с акцепторной группировкой. |
| Date | |
| Source | Huang C. L.-H. Roger Yonchien Tsien. 1 February 1952 — 24 August 2016 // Biogr. Mems Fell. R. Soc., 2018, v. 65, p. 405-428. |
| Author | Christopher L.-H. Huang |
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