File:American malacological bulletin (1986) (18152798802).jpg

Title: American malacological bulletin
Identifier: americanmal4519861987amer (find matches)
Year: 1983 (1980s)
Authors: American Malacological Union
Subjects: Mollusks; Mollusks
Publisher: (Hattiesburg, Miss. ?) : (American Malacological Union)
Contributing Library: Smithsonian Libraries
Digitizing Sponsor: Biodiversity Heritage Library

View Book Page: Book Viewer
About This Book: Catalog Entry
View All Images: All Images From Book
Click here to view book online to see this illustration in context in a browseable online version of this book.

Text Appearing Before Image:
EYSTER: NUDIBRANCH EMBRYONIC CAPSULES 207
Text Appearing After Image:
Figs. 2, 3. Transmission electron micrographs of sperm inside Aeolidia papulosa capsules (C) 14 days after capsule deposition. The 9 + 2 arrangement of microtubules (arrowheads) is still detectable, as is the periaxonemal sheath and keel (*). Glycogen is not detected in the lumen of the keel (*). Fig. 2. Standard TEM preparation followed by staining with uranyl acetate and lead citrate. Fig. 3. Standard TEM preparation followed by staining for periodate-reactive carbohydrates (arrows). Bar = 0.2 /im for both. varying lengths of time from about 1-8 weeks depending on the temperature, the developmental pattern of the species, and various other factors associated with hatching. The organism that hatches from each capsule is either a free- swimming veliger larva or a crawling juvenile, depending on the species. Hatching is discussed below. In addition to eggs, each capsule encloses multiple sperm (Figs. 2, 3). In nudibranchs, fertilization usually occurs inside the parent soon after gamete mixing (Schemekel, 1971). The fate of the supernumerary sperm is unknown. In some species, such as Archidoris pseudoargus, intracapsular sperm are not detected after oviposition, presumably because they are somehow readily degraded (Thompson, 1976). In other species they are visible and are capable of occasional movement several days after oviposition (Thompson, 1976; pers. observ.) In transmission electron microscopy (TEM) sec- tions, sperm are occasionally observed fortuitously (Figs. 2, 3, 6). The sperm were visible with light microscopy within the capsules of Tritonia hombergi up to 14 days after oviposition (Thompson, 1976) and were detectable with TEM in Cor- yphella salmonacea capsules 50 days after oviposition (Fig. 6). The energy reserve of the sperm, glycogen-like particles in the helical keel (Anderson and Personne, 1976; Eckelbarger and Eyster, 1981), were not detected in Aeolidia papulosa sperm at 14 days (5°C) after oviposition (Figs. 2, 3) or in Coryphella salmonacea sperm at 50 days (5-8°C) after oviposition (Fig. 6). In one section subjected to PA-TSC-SP (periodic acid, thiosemicarbazide, silver proteinate) staining

Note About Images