File:3D-SIM-1 NPC Confocal vs 3D-SIM.jpg
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[edit]Description3D-SIM-1 NPC Confocal vs 3D-SIM.jpg |
English: Comparison of resolution obtained by confocal laser scanning microscopy (clsm, left) and 3D structured illumination microscopy (3D-SIM-Microscopy, right). Top images each show a nucleus of a mouse cell, the white boxes are magnified at the bottom. Nuclear pores (anti-NPC, red) nuclear envelope (anti-Lamin, green). Chromatin/DNA (DAPI, blue). scale bars: top 5 µm, bottom 1µm.
For further information see: Schermelleh L, Carlton PM, Haase S, Shao L, Winoto L, Kner P, Burke B, Cardoso MC, Agard DA, Gustafsson MG, Leonhardt H, Sedat JW (June 2008). "Subdiffraction multicolor imaging of the nuclear periphery with 3D structured illumination microscopy". Science (journal) 320 (5881): 1332–6. DOI:10.1126/science.1156947. PMID 18535242.
Deutsch: Vergleich des Auflösungsvermögen von konfokaler Laser-Scanning Mikroskopie (CLSM, links) und 3D-SIM (rechts). Zellkernporen (anti-NPC, rot), Zellkernhülle (anti-Lamin B, grün), sowie DNA verpackt in Chromatin (DAPI, blau) wurden in einer Mauszelle simultan angefärbt. Der Maßstab entspricht 5 µm (oben) und 1 µm (unten). Für weitere Informationen siehe oben zitierte Veröffentlichung.) |
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Date | |||
Source | Lothar Schermelleh | ||
Author | Lothar Schermelleh | ||
Permission (Reusing this file) |
This file is licensed under the Creative Commons Attribution-Share Alike 3.0 Unported license.
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3D-SIM images
[edit]Annotations InfoField | This image is annotated: View the annotations at Commons |
scale bar: 5 µm
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 17:21, 16 January 2009 | 1,069 × 848 (307 KB) | Dietzel65 (talk | contribs) | {{Information |Description={{en|1=(to be added soon)}} {{de|1=Vergleich für das Auflösungsvermögen von konfokaler Laser-Scanning Mikroskopie (CLSM, links) und 3D-SIM (rechts). Zellkernporen (NPC, rot), Zellkernhülle (Lamin B, grün), sowie DNA |
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Orientation | Normal |
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Horizontal resolution | 300 dpi |
Vertical resolution | 300 dpi |
Software used | Adobe Photoshop CS3 Windows |
File change date and time | 10:45, 15 January 2009 |
Color space | sRGB |
Image width | 1,069 px |
Image height | 848 px |
Date and time of digitizing | 11:45, 15 January 2009 |
Date metadata was last modified | 11:45, 15 January 2009 |
IIM version | 111 |